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Bioinformatic and Biochemical Characterizations of C-S Bond Formation and Cleavage Enzymes in the Fungus Neurospora Crassa Ergothioneine Biosynthetic Pathway

Once water is added, hydrolysis, oxidation, aggregation, and contamination risk become more important

Including biotin in your culture and inducing protein expression with IPTG results in in vivo biotinylation

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